ifn gamma duo set kit  (R&D Systems)

Journal: Immune Network

Article Title: The Immunosuppressive Potential of Cholesterol Sulfate Through T Cell Microvilli Disruption

doi: 10.4110/in.2023.23.e29

Figure Lengend Snippet: (A) Schematic diagram of AD challenge in recipient mice. (B, C) CD3 + T cells from PBS or CS-injected mice isolated from TNCB-sensitized donor mice, followed by treatment of the ears of recipient mice with TNCB and mite after transfer. At day 7, and ear vein and colour were analysed in photographs and ear swelling was determined. Data are shown as the mean ± SEM (n=7) and are representative of 4 experiments. (D) IL-2 and IFN-γ expression in serum from AD-induced recipient mice (day 7). (E) CD69/25 expression for CD3 + T cells isolated from AD-induced recipient mice (day 7). Isolated CD3 + T cells from the recipient mice were stimulated on α-CD3/28 coated plate for 8 h, and CD69/25 expression was measured by using flow cytometry. Data are presented as mean ± SD. ** p<0.01.

Article Snippet: The amounts of IL-2 and IFN-γ in the serum for each condition were determined by ELISA with Duo Set Mouse ELISA kits for IL-2 and IFN-γ (R&D Systems) according to the manufacturer’s instructions.

Techniques: Injection, Isolation, Expressing, Flow Cytometry